Crystallization and diffraction of an Isl1-Ldb1 complex.
نویسندگان
چکیده
A stable intramolecular complex comprising the LIM domains of the LIM-homeodomain protein Isl1 tethered to a peptide region of Ldb1 has been engineered, purified and crystallized. The orthorhombic crystals belonged to space group P222(1), with unit-cell parameters a=57.2, b=56.7, c=179.8 Å, and diffracted to 3.10 Å resolution.
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متن کاملCrystallization of an Lhx3-Isl1 complex.
A stable intramolecular complex comprising the LIM domains of the LIM-homeodomain protein Lhx3 tethered to a peptide region of Isl1 has been engineered, purified and crystallized. The monoclinic crystals belong to space group C2, with unit-cell parameters a = 119, b = 62.2, c = 51.9 A, beta = 91.6 degrees , and diffract to 2.05 A resolution.
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ورودعنوان ژورنال:
- Acta crystallographica. Section F, Structural biology and crystallization communications
دوره 68 Pt 11 شماره
صفحات -
تاریخ انتشار 2012